2 edition of LCK promoter switch requirements during thymocyte development. found in the catalog.
LCK promoter switch requirements during thymocyte development.
Michael James Parsons
Thesis (M.Sc.) -- University of Toronto, 1995.
|Series||Canadian theses = -- Thèses canadiennes|
|The Physical Object|
|Pagination||2 microfiches : negative. --|
Publication. Control of thymocyte development and recombination-activating gene expression by the zinc finger protein Zfp Zhang F, Thomas LR, Oltz EM, Aune TM Nat Immunol. 7 (12): PMID: DOI/ni Chapter 22 Using the Zebrafish Model to Study T Cell Development Yong Zhang and David L. Wiest Abstract While zebrafish have for some time been regarded as a powerful model organism with which to study early events in hematopoiesis, recent evidence suggests that it also ideal for unraveling the molecular requirements for T cell development in the thymus.
Lck is a Src-family tyrosine kinase whose activity is essential for T cell activation and development. The phosphorylation of two key tyrosine residues in Lck, Tyr and Tyr , regulate its activation by inducing profound changes in conformation. In vitro, CD45, a leukocyte-specific tyrosine phosphatase, preferentially dephosphorylated Tyr , as opposed to Tyr . The molecular basis for T cell receptor signaling events that lead to differential cellular immune responses has been a fundamental question in immunology. For example, the molecular mechanism governing positive and negative T cell selection during thymocyte development has remained elusive. Furthermore, the mechanisms governing variable T cell receptor-mediated .
contents in brief. 1 overview of the immune system, 1. 11 activation and function of t cells, 2 innate immunity, 12 cytokines, 3 adaptive immunity, points of thymocyte di erentiation (reviewed in [ ]). In this context, we previously reported that Inhibins are the major Activin ligands expressed in the thymus [ ]. Moreover, both Inhibins and Activins promote DN to DP thymocyte di erentiation during in vitro T cell development of murine fetal thymocytes. In contrast, Activins, but not Inhibins.
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Amy Y. Chow, Ira Mellman, in Measuring Immunity, T CELL SELECTION. During thymocyte development, MHCII on thymic cortical epithelium mediates engagement of the T-cell receptor (TCR) on CD4+ cells thereby promoting selection.
Negative selection, or the elimination of thymocytes, occurs when a high affinity interaction occurs between MHCII-peptide and the. The Lck-Cre transgenic mouse uses the proximal promoter of the Lck (lymphocyte protein tyrosine kinase) gene, which is first expressed early in thymocyte development at the double negative stage.
After T cells fully mature, the level of expression of this transgene decreases by approximately 10 fold. We have investigated the potential role of the Lck tyrosine kinase in regulating intracellular signaling during thymocyte development.
While Lck is known to be critical for initial T-cell receptor signaling events, it may have an independent role in regulating intracellular signaling through the function of its SH3 by: Promoter switching in Epstein-Barr virus during the initial stages of infection of B lymphocytes.
Proc Natl Acad Sci U S A. Mar; 87 (5)– [PMC free article] Corbin V, Maniatis T. Role of transcriptional interference in the Drosophila melanogaster Adh promoter switch.
Nature. Jan 19; ()– A Thymocyte is an immune cell present in the thymus, before it undergoes transformation into a T cell. Thymocytes are produced as stem cells in the bone marrow and reach the thymus via the blood.
Thymopoiesis describes the process which turns thymocytes into mature T cells according to either negative or positive selection.
This selection process is vitally important in shaping. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.
Recently, Strauss and colleagues (8) showed a requirement for the Lck SH3 domain in optimal T-cell development. In Lck W97A SH3 domain knockin mice. Because c-Myb plays critical roles during thymocyte developm we bred a conditional knockout model based on a tamoxifen-regulated form of Cre (Cre-ER T2) 23.
TCR repertoire is regulated sequentially by three major factors: VDJ recombination, thymic selection, and peripheral homeostasis.
Using mice with Lck-cre-driven conditional deletion of PP4 locus, we show that protein phosphatase 4 (PP4), the product of PP4 gene, is essential for the development of mature T cells, such that thymocyte development is completely blocked at the Author: Ching-Yu Huang, En-Wei Hsing, Fang-Hsuean Liao, Wan-I Hsou, Yu-Jun Lin, Yi-Jyun Jhou.
Alternative promoters usage is an important paradigm in transcriptional control of mammalian gene expression. However, despite the growing interest in alternative promoters and their role in genome diversification, very little is known about how and on what occasions those promoters are differentially regulated.
Runx1 transcription factor is a key regulator of early Cited by: title = "Regulation of SATB1 during thymocyte development by TCR signaling", abstract = "T lymphocyte development and differentiation is a multi-step process that begins in the thymus and completed in the by: 8.
Whilst many of the developmental processes occurring during thymocyte development have become elucidated over the past several years, systems directed towards modelling the dynamic migratory patterns and real-time cellular interactions will radically advance our understanding of how such pivotal cells of the immune system are generated.
of mice that express the Cre recombinase at late stages of thymocyte development are of value for determining the impact of mutations on T cell function in the absence of complicating effects on early thymocyte selection. The Journal of Immunology,– T he development of T lymphocytes is a well described multistage process.
strate that E2A functions as a regulator of thymocyte positive selection. Key words: E2A • positive selection • thymocyte development • helix-loop-helix D uring maturation in the thymus, T lineage cells inter-act with peptide containing MHC proteins either to differentiate or, alternatively, to undergo programmed cell death.
*A thymocyte that does not receive a signal dies by apoptosis and is removed by macrophages. T-cells that can recognize self-MHC are positively selected in the thymus -Self-peptides presented in the MHC molecules of cortical epithelial cells are derived from self-proteins present in.
If a double-negative thymocyte has just completed a productive β-chain gene rearrangement, which of the following describes the immediate next step in the development of this thymocyte. A pre-T-cell receptor is assembled as a superdimer.
Rearrangement of γ- and δ-chain genes commences. Expression levels of RAG-1 and RAG-2 are. The E-protein transcription factors E2A and HEB function in a lineage- and stage-specific manner to orchestrate many critical events throughout lymphocyte development.
The function of E-proteins in both B- and T-lymphocyte development has been extensively studied through the use of single-gene knockout animals. Unlike B cells, which rely primarily on E2A Cited by: For Immunology Chapter 9 Day 1 What role(s) does Nothch1 play in early thymocyte development.
Draw a thymus (figure d). Add all of the information from table to your drawing. Why are thymocytes more likely to develop into αβ T cells than γδ T cells.
What are some ways in which γδ T cells fall into the innate, rather than. Cell Receptor Thymocyte Development Immature Thymocyte Human Thymocyte Thymus Lobe These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm by: 3.
Development of temporal structure in zebra finch song Evidence that the limb bud ectoderm is required for survival of the underlying mesoderm. Influence of testosterone on cell proliferation in the telencephalic ventricle zone.
Gene Expression Program Lineage Commitment Thymocyte Development Thymocyte Differentiation Thymocyte Subset These keywords were added by machine and not by the authors. This process is experimental and the keywords may Cited by: 5.Tai et al. used both Cd2 and Lck promoter to express cFLIP L in T cells.
In contrast, Oehme et al. generated transgenic mice expressing human cFLIP S in T cells under Lck promoter. These transgenic T cells were all resistant to CDinduced apoptosis, whereas activation-induced cell death was not by: Apoptosis of thymocytes is involved in the negative selection of thymus, but it remains unclear how the cell death of thymocytes is regulated by the thymic stromal cells.
By using immunohistochemistry, TdT-mediated dUTP nick end labeling (TUNEL) and flow cytometry methods, it was found that Fas ligand was expressed in the thymic medulla and a mouse Cited by: 4.